Alkaline Protease CAS NO.9014-01-1

The Alkaline Protease is a solid enzyme produced by submerged fermentation from Bacillus licheniformis according to the specification of GB/T23527-2009 (China). It is widely applied in detergent, leather depilation and softening, silk degumming, Heparin Sodium, Chondroitin and etc.

Features:

a. Simplify production process and reduce production period.

b. Improve end product quality, leather yield and reduce production cost.

c. No pollution to the equipment, environment and leather.

d. Environmental-friendly & bio-degradation

1. Application in Heparin Sodium:

(1).Add salt and alkali at earlier stage. The process is the same with salt dissolving.

(2).Rise the low temperature to 65°C and keep 1 hour.

(3).Cool down the temperature to 45°C and add 3g enzyme powder (100,000 u/g) for one piece of small intestine.

(4).Rise the temperature to 60°C after adding the enzyme and keep 4-6 hours.

(5).Filtering after rising the temperature to 80°C.

(6).Add Resin when the temperature is cooled down to 60°C after the filtering.

(7).The procedure of dissolving salt is different.

Adjust the dosage and the enzymolysis condition according to the quality of raw materials and the practical production conditions.

2. Application in Chondroitin

(1).Stir the enzyme well with water, and then add it into the materials.

(2).Add 0.4%-0.6% of enzyme powder during the whole process of enzymolysis dissolution. Add the enzyme separately by twice. Add 60-70% of the total enzyme in the front two thirds time and add the rest enzyme during the left time.

(3).Enzymolysis condition: Adjust the temperature to 45-60°C and pH at 10.5. Process time: 5-8 hours. Stir once every 30mins.

Adjust the dosage and the enzymolysis condition according to the quality of raw materials and practical production condition.

3. Pretreatment in silk degumming:

Pretreatment → Dehydration → acid treatment (add protease 18-24 u/ml and soap 0.75% at 48-50°C and pH 10.5) → Dehydration → skin tearing off → washing →Dehydration → fluffing → drying → fluffing

4. Application in leather depilation:

Raw Hides → washing → degreasing → alkali expansion → feather plucking → splitting → dealkalization → enzyme depilation → washing → pickling →tanning.