Guanidine thiocyanate 593-84-0nucleic acid testing CAS NO.593-84-0
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- Min.Order: 1 Kilogram
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- Product Details
Keywords
- Guanidine thiocyanate
- 593-84-0
- anti-virus
Quick Details
- ProName: Guanidine thiocyanate 593-84-0nucleic ...
- CasNo: 593-84-0
- Molecular Formula: C2H6N4S
- Appearance: crystalline powder
- Application: Uses strong denaturant agent, mainly u...
- DeliveryTime: 7day
- PackAge: 25KG
- Port: shanghai
- ProductionCapacity: 3 Metric Ton/Day
- Purity: 99.5%
- Storage: Dry and ventilated
- Transportation: expressage
- LimitNum: 1 Kilogram
- Impurity: not have
- molecular weight: 118.16100
Superiority
Hebei Yanxi Chemical Co., Ltd. is a professional research and development of the production of lead acetate, 2-phenylacetamide Guanidine thiocyanate carbomer enterprises.The backbone members of the company are established by local well-known entrepreneurs and professional engineers under the call and support of the Party's "low carbon, environmental protection, safety and stability". The company has made bold innovation in traditional production equipment and technology, and made use of "high temperature heat dissipation" and "plastic basin crystallization" technology to make the product quality reach the same industry on the basis of reducing the product cost.With the efforts of all the staff of the company, we have established many partners in Shandong, Henan, Guangdong and other places in the past decades. Under the close supervision of the quality inspection department of the company, we implement production and sales by "labeling" method, providing the most convenient and fastest service for users all over the country.
Companies in the "advance with The Times, and the city every day, with the advancement of" under the guidance of the core ideas, adhering to the "relying on scientific and technological innovation, the development of circular economy" of the enterprise purpose, established in the market, enhance strength, go the way of science and environmental sustainable development, relying on the national technology research and development center, increasing spending on research and development based on the domestic market, expand the international market, manufacturing high quality products, sincere service society, toward a modern, ecology, science and technology, the chemical enterprises of the world.
Details
Guanidinium thiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used in the extraction of DNA and RNA.
Note: this compound may also be recognized as guanidine thiocyanate. This is because guanidinium is the conjugate acid of guanidine and is called the guanidinium cation, [CH6N3]+.
Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus that caused the 1918 "Spanish flu", so that it can be studied safely. This application is presumably based on its denaturation activity.
Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them. These enzymes would otherwise damage the extract.
A commonly used method is guanidinium thiocyanate-phenol-chloroform extraction. It is not strictly necessary to use phenol or chloroform if extracting RNA for Northern blotting or DNA for Southern blot analysis because the gel electrophoresis followed by transfer to a membrane will separate the RNA/DNA from the proteins. Additionally, since these methods use probes to bind to their conjugates, peptides that get through the process don't generally matter unless a peptide is an RNase or DNase, and then only if the enzyme manages to renature, which should not occur if proper protocols are followed. A possible exception might be when working with temperature extremophiles because some enzymes of these organisms can remain stable under extraordinary circumstances.