Isopropyl-beta-D-thiogalactopyranoside CAS NO.367-93-1

Product Name: IPTG

Synonyms: Isopropyl-β-d-thiogalactopyranoside  

CAS:367-93-1

Struture:

Molecular Formula: C9H18O5S

Molecular Weight:  238.3

Assay:   ≥98.0 %  

Property:white powder, soluble in water is clear and colourless liquid,Freezing preservation temperature: 2-8

° C, make good save in - 20 ° C, after a month can be placed at room temperature.Induced the expression of exogenous gene, widely used in prokaryotic expression system, make its expression quantity increased, product stability, is easy to identify, the advantages of easy purification

A very strong inducer, it is very stable even in bacterial metabolism, so it is widely used in laboratories. Isopropyl glucosinolates galactose glucoside (IPTG) is a kind of extremely strong inducer, not by bacteria metabolism and is very stable, so widely used by the laboratory IPTG is beta - galactose glucoside enzyme activity of inductive substance. Based on this feature, as the carrier of pUC series of DNA (or other carrier with lacZ gene DNA) with lacZ lack for host cell transformation, or by the carrier of M13 phage DNA transfection, if add X - Gal in plate culture medium and IPTG, due to the alpha beta - galactose glucoside enzyme - complementary, can according to whether the present white colonies (or plaque) and easily pick out gene recombinant. In addition, it also can be used as a lac or tac promoter expression vector expression of the inducer to use.